Cell Density Calculator:
Enter the values of number of cells per square, AC(cells), dilution factor, DF and volume of one cell, VC(mL) to determine the value of Cell density, CD(cells/mL).
Cell Density Formula:
Cell density measures the number of cells in each volume of a medium, commonly expressed in cells per millilitre (cells/mL).
Higher cell density indicates a greater concentration of cells within a culture, which can affect the growth rate and health of the culture. The density is influenced by factors like nutrient availability, growth conditions, and the health of the cells.
The dilution factor (DF) is the ratio of the original volume to the volume after dilution. A higher dilution factor indicates a more diluted sample.
A specific volume (VC) is taken from the diluted mixture for cell counting using a counting chamber or other methods.
A sample of the cell suspension is obtained. If the cell concentration is high, the sample might be diluted with a known volume of liquid to create a more manageable concentration for counting. The dilution factor (DF) is calculated.
A specific volume (VC) of the diluted suspension is used for cell counting. The actual number of cells counted (AC) in this volume (VC) is multiplied by the dilution factor (DF) to account for the dilution and determine the total number of cells in the original volume. This final value is the cell density (CD).
Cell density, CD(cells/mL) in cells per millilitre is calculated by dividing the product of average number of cells per square, AC(cells), dilution factor, DF by volume of one cell, VC(mL) in millilitre.
Cell density, CD(cells/mL) = AC(cells) * DF / VC(mL)
CD(cells/mL) = cell density in cells per millilitre, cells/mL.
AC(cells) = average number of cells per square, cells.
DF = dilution factor.
VC(mL) = volume of one cell in millilitre, mL.
Cell Density Calculation:
- Find cell density:
Given: AC(cells) = 0.8, DF = 10, VC(mL) = 2mL.
Cell density, CD(cells/mL) = AC(cells) * DF / VC(mL)
CD(cells/mL) = 0.8 * 10 / 2
CD(cells/mL) = 4cells/mL.
- Find the volume:
Given: AC(cells) = 1.2, DF = 5, CD(cells/mL) = 3cells/mL.
Cell density, CD(cells/mL) = AC(cells) * DF / VC(mL)
VC(mL) = AC(cells) * DF / CD(cells/mL)
VC(mL) = 1.2 * 5 / 3
VC(mL) = 2mL.